anti sdhc  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Iron addicted colorectal cancers exploit Heme-Complex II axis to resist oxidative cell death

doi: 10.1101/2025.11.05.686813

Figure Lengend Snippet: A) Schematic showing the xenograft tumor model with subcutaneous implantation. B) Tumor weight from B) C) Lipid ROS are measured by C11-BODIPY581/591 in EPCAM positive cells from the tumors from B). D) Schematic showing breeding strategy to generate tamoxifen inducible intestine specific SDHC deficient mice. E) Western blot images showing cellular and mitochondrial protein expression of SDHC 5days post tamoxifen induction via 100mg/kg body weight I.P. injection in WT ( Sdhc +/+), Het ( Sdhc +/-) and KO ( Sdhc -/-) mice. F) Representative H&E images of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. G) Inflammatory histology scored for small intestine and colon of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. H) Representative immunohistochemistry images showing accumulation of 4 hydroxynonenal (4HNE) in small intestine and colon of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. I) Schematic showing treatment protocol for colitis associated cancer model using AOM/DSS in Sdhc F/F (WT) and Sdhc Het IE (Het) mice. J) Tumor number measured in mice from I). K) Tumor burden measured in mice from I) L) Lipid ROS measured by C11-BODIPY581/591 in EPCAM positive epithelial cells derived from I). M) Schematic showing role of heme-SDHC-CoQ axis in mitigating iron induced cell death in CRC cells. All data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons test for (B) and (G) and t test for (C), (J), (K) and (L). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Followed by blocking with 5% goat serum in PBS and sections were then probed with primary antibody against SDHC (Proteintech, 14575-AP) or 4HNE (R&D Systems, MAB3249-SP) diluted at 1:500 in the blocking solution.

Techniques: Western Blot, Expressing, Injection, Immunohistochemistry, Derivative Assay

Journal: bioRxiv

Article Title: Iron addicted colorectal cancers exploit Heme-Complex II axis to resist oxidative cell death

doi: 10.1101/2025.11.05.686813

Figure Lengend Snippet: A) Western blot images showing protein expression of SDHA, SDHB and beta actin in SDHA and SDHB knockdown HT29 cells via constitutive shRNA expression. B) Representative crystal violet staining of HT29 SDHA and SDHC knockdown cells from A) 14 days post copper sulphate (100μM) + elesclomol (Cu-Es), C) hydrogen peroxide (H 2 O 2 ) and D) 5-fluoroUracil (5FU) at indicated concentrations. E) Heatmap representation of relative colony count from B). F) Representative crystal violet staining images 14 days post FAC (at indicated concentrations), dimethyl succinate, dimethyl malonate, and diethyl malonate. G) Representative crystal violet staining image of NDUSF1 and 2 knockdown cells 14 days post FAC treatment (0.25, 0.5mM) with or without SA (250μM) or heme (10μM).

Article Snippet: After lysis solubilized proteins were resolved on 12% SDS-polyacrylamide gels and transferred to PVDF membrane, blocked with 5% milk in TBST and probed overnight with indicated primary antibodies diluted at 1:1000 in the blocking buffer for GPX4 (Proteintech, 67763-1-Ig), FTH1 (Cell Signaling,3998S), UROD (Santa Cruz, SC-365297), FECH (Proteintech, 14466-1-AP), SDHA (Cell Signaling, 11998), SDHB (Proteintech, 10620-1-AP), SDHC (Proteintech, 14575-AP), β-actin (Proteintech 66009-1-Ig).

Techniques: Western Blot, Expressing, Knockdown, shRNA, Staining